Lm. Graves et al., COMPARISON OF RIBOTYPING AND MULTILOCUS ENZYME ELECTROPHORESIS FOR SUBTYPING OF LISTERIA-MONOCYTOGENES ISOLATES, Journal of clinical microbiology, 32(12), 1994, pp. 2936-2943
Ribotyping was compared with multilocus enzyme electrophoresis (MEE) f
or subtyping 305 Listeria monocytogenes isolates from clinical and non
clinical sources. For ribotyping, EcoRI-restricted genomic DNA fragmen
ts of L. monocytogenes strains were separated by agarose gel electroph
oresis, and Southern blots were probed with a cloned Escherichia coli
rrnB operon (plasmid pKK3535) labeled with digoxigenin. The L. monocyt
ogenes isolates were divided into 28 distinct ribotypes, while MEE ana
lysis divided the same isolates into 78 electrophoretic types (ETs). O
n the basis of their ribotype profiles, the strains were divided into
two subgroups. The ribotype alpha (RT alpha) subgroup contained seroty
pes 1/2a. 1/2c, and 3a, and the ribotype beta (RTP) subgroup contained
serotypes 1/2b, 3b, 4b, and 4ab. This division is in complete agreeme
nt with MEE analysis, which divides the species into two subgroups (ET
groups A and B), with the same serotype distribution in each subgroup
. Overall, MEE was more discriminating than ribotyping. However, in se
veral instances ribotyping discriminated between isolates within the s
ame ET. Ribotyping was more discriminating for serotypes 1/2a, 1/2c, a
nd 3a (Simpson's Index for Diversity [DI] = 0.81) than for serotypes 1
/2b and 4b (DI = 0.76). A substantial proportion (69%) of serotype 1/2
b and 4b strains clustered in five ETs and five ribotypes. These data
suggest that ribotyping and MEE do not provide adequate discrimination
between strains of serotypes 1/2b and 4b. Methods such as pulsed-fiel
d gel electrophoresis and random amplified polymorphic DNA analysis sh
ould be explored for further discrimination of strains of these seroty
pes.