USE OF GEN-PROBE ACCUPROBES TO IDENTIFY MYCOBACTERIUM-AVIUM COMPLEX, MYCOBACTERIUM-TUBERCULOSIS COMPLEX, MYCOBACTERIUM-KANSASII, AND MYCOBACTERIUM-GORDONAE DIRECTLY FROM BACTEC TB BROTH CULTURES
Bs. Reisner et al., USE OF GEN-PROBE ACCUPROBES TO IDENTIFY MYCOBACTERIUM-AVIUM COMPLEX, MYCOBACTERIUM-TUBERCULOSIS COMPLEX, MYCOBACTERIUM-KANSASII, AND MYCOBACTERIUM-GORDONAE DIRECTLY FROM BACTEC TB BROTH CULTURES, Journal of clinical microbiology, 32(12), 1994, pp. 2995-2998
To evaluate the utility of Gen-Probe AccuProbes for the identification
of mycobacteria directly from BACTEC TB 12B vials containing acid-fas
t bacilli, culture results for 11,375 clinical specimens other than bl
ood received from 1 January 1992 to 30 September 1993 were reviewed re
trospectively. During this period, a total of 359 of 11,375 BACTEC via
ls were positive for acid-fast bacilli and were evaluated for mycobact
eria with one or more probes: 224 were probed for Mycobacterium comple
x, 253 were probed for Mycobacterium avium complex, 64 were probed for
Mycobacterium kansasii, and 77 were probed for Mycobacterium gordonae
. After initial testing with the probes, 75 vials were positive for M.
tuberculosis complex, 99 were positive for M. avium complex, 11 were
positive for M. kansasii, and 55 were positive for M. gordonae. Repeat
testing of vials that were initially probe negative or testing of col
onies from subcultures of these vials identified an additional 11 M. t
uberculosis, 27 M. avium complex, 1 M. kansasii, and 9 M. gordonae tha
t were not detected on initial screening. On the basis of these data,
the percentage of organisms identified directly from the BACTEC TB 12B
vials upon initial screening with each of the four AccuProbes was 87.
2% for M. tuberculosis complex, 78.6% for M. avium complex, 91.7% for
M. kansasii, and 85.9% for M. gordonae.