OPTIMIZATION OF QUANTITATIVE CULTURE ASSAY FOR HUMAN-IMMUNODEFICIENCY-VIRUS FROM PLASMA

Authors
LATHEY JL FISCUS SA RASHEED S KAPPES JC GRIFFITH BP ELBEIK T SPECTOR SA REICHELDERFER PS
Citation
Jl. Lathey et al., OPTIMIZATION OF QUANTITATIVE CULTURE ASSAY FOR HUMAN-IMMUNODEFICIENCY-VIRUS FROM PLASMA, Journal of clinical microbiology, 32(12), 1994, pp. 3064-3067
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
32
Issue
12
Year of publication
1994
Pages
3064 - 3067
Database
ISI
SICI code
0095-1137(1994)32:12<3064:OOQCAF>2.0.ZU;2-4
Abstract
Experimental conditions essential to a simple, reproducible, quantitat ive human immunodeficiency virus plasma assay were determined. Five pa rameters were evaluated: length of culture, reproducibility, assay dil ution schema, washing procedures, and anticoagulant usage. The recomme nded quantitative plasma assay utilizes undiluted citrated plasma cult ured with peripheral blood mononuclear cells for 14 days with fivefold dilutions and a medium change on day 1 with no washing.