EVALUATION OF NEW TRANSPORT MEDIUM FOR DETECTION OF HERPES-SIMPLEX VIRUS BY CULTURE AND DIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY

Authors
OGBURN JR HOFFPAUIR JT COLE E HOOD K MICHAEL D NGUYEN T RADEN S RAJU B REISINGER V OEFINGER PE
Citation
Jr. Ogburn et al., EVALUATION OF NEW TRANSPORT MEDIUM FOR DETECTION OF HERPES-SIMPLEX VIRUS BY CULTURE AND DIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY, Journal of clinical microbiology, 32(12), 1994, pp. 3082-3084
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
32
Issue
12
Year of publication
1994
Pages
3082 - 3084
Database
ISI
SICI code
0095-1137(1994)32:12<3082:EONTMF>2.0.ZU;2-O
Abstract
The transport medium Multi-Microbe Media (M4) was evaluated prospectiv ely by culture and direct enzyme-linked immunosorbent assay (ELISA) fo r detection of herpes simplex virus from 473 specimens. In addition, 3 77 specimens in Bartels Viral Transport Medium were evaluated. By usin g culture as a ''gold standard,'' the ELISA sensitivity was approximat ely 85%, while the specificities exceeded 96% for both media.