Onychophorans possess a combination of annelidan and arthropodan chara
cters and are always prominent in discussions of arthropod evolution.
The chitinous cuticle is moulted periodically in juveniles as well as
in adults. Using radioimmunoassay (RIA) and high performance liquid ch
romatography (HPLC), the presence of free and conjugated ecdysteroids
is reported in the body wall, digestive tract, slime gland and ovary/u
terus of adult females of Euperipatoides leuckartii. Within free ecdys
teroids, ecdysone is dominant. The functional significance of ecdyster
oids is discussed in the light of the evolutionary history of moulting
hormones.