STIMULATION OF VACCINIA TOPOISOMERASE-I BY NUCLEOSIDE TRIPHOSPHATES

Authors
SEKIGUCHI J SHUMAN S
Citation
J. Sekiguchi et S. Shuman, STIMULATION OF VACCINIA TOPOISOMERASE-I BY NUCLEOSIDE TRIPHOSPHATES, The Journal of biological chemistry, 269(47), 1994, pp. 29760-29764
Citations number
17
Categorie Soggetti
Biology
Journal title
The Journal of biological chemistry → ACNP
ISSN journal
00219258
Volume
269
Issue
47
Year of publication
1994
Pages
29760 - 29764
Database
ISI
SICI code
0021-9258(1994)269:47<29760:SOVTBN>2.0.ZU;2-S
Abstract
The rate of relaxation of supercoiled DNA by purified vaccinia topoiso merase I is stimulated 20-fold by 5 mM ATP. A similar effect is elicit ed by GTP, CTP, UTP, dATP, and adenosine 5'-(beta,gamma-imido)triphosp hate. ATP-mediated rate enhancement requires salt as a coactivator. AD P and inorganic pyrophosphate also stimulate relaxation 10-20-fold, wh ereas AMP and inorganic phosphate have little effect. A model for allo steric activation of topoisomerase by nucleotides is suggested.