OLIGOTYPING OF HLA-A2, HLA-A3, AND HLA-B44 SUBTYPES - DETECTION OF SUBTYPE INCOMPATIBILITIES BETWEEN PATIENTS AND THEIR SEROLOGICALLY MATCHED UNRELATED BONE-MARROW DONORS
Jm. Tiercy et al., OLIGOTYPING OF HLA-A2, HLA-A3, AND HLA-B44 SUBTYPES - DETECTION OF SUBTYPE INCOMPATIBILITIES BETWEEN PATIENTS AND THEIR SEROLOGICALLY MATCHED UNRELATED BONE-MARROW DONORS, Human immunology, 41(3), 1994, pp. 207-215
We have set up a simple PCR-SSO oligotyping procedure that is able to
discriminate ten HLA-A2 (2 PCR/11 probes), two HLA-A3 (1 PCR/1 probe),
and two HLA-B44 subtypes (1 PCR/2 probes). The frequency of these sub
types has been determined in a large panel of local blood donors and l
eukemic patients in combination with their unrelated potential donors.
A0201 and A*0301 were the predominant subtypes (> 95%) for A2 and A3
, respectively. B4402 occurred twice as frequently as B*4403. A2 and
B44 subtype mismatches were analyzed in a group of 30 patients and the
ir 116 unrelated potential donors who were matched serologically (low-
stringency matching: AB without splits, DR1-10). For seven patients (2
1%) at least one A2- or B44-subtype-mismatched donor was found. For tw
o of these patients (7%), the subtype-mismatched donor would have been
considered as compatible on the basis of high stringency matching (AB
splits, DRB1 subtypes, DRB3/B5). For one patient of Mediterranean ori
gin, all five donors recruited from a north European registry (matched
with high stringency) appeared to be subtype incompatible (A0201/A*0
205). The rather low percentage of A2- and B4-subtype mismatches in DR
B1/B3/B5 matched combinations confirms the significance of linkage dis
equilibria of HLA antigens. Because unrelated donor selection is done
through international registries, however, class I subtyping might be
necessary when individuals originate from different geographic areas.