QUANTITATIVE-ANALYSIS OF SUPEROXIDE ANION GENERATION IN LIVING CELLS BY USING CHEMILUMINESCENCE VIDEO MICROSCOPY

Authors
SUZAKI E KAWAI E KODAMA Y SUZAKI T MASUJIMA T
Citation
E. Suzaki et al., QUANTITATIVE-ANALYSIS OF SUPEROXIDE ANION GENERATION IN LIVING CELLS BY USING CHEMILUMINESCENCE VIDEO MICROSCOPY, Biochimica et biophysica acta (G). General subjects, 1201(2), 1994, pp. 328-332
Citations number
21
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta (G). General subjects → ACNP
ISSN journal
03044165
Volume
1201
Issue
2
Year of publication
1994
Pages
328 - 332
Database
ISI
SICI code
0304-4165(1994)1201:2<328:QOSAGI>2.0.ZU;2-4
Abstract
Superoxide anions (O-2(-)) generated by rabbit neutrophils were detect ed and quantified by a video microscope equipped with a photon-countin g camera. One count obtained by this system was equivalent to 59 amol of O-2(-). Maximum O-2(-) production was observed at 6-8 min after sti mulation and was estimated as 1.9 fmol/min per cell on the average.