PHYSICAL MAPPING OF THE SCHIZOSACCHAROMYCES-POMBE HISTONE GENES

The histone-encoding genes in Schizosaccharomyces pombe were physicall y mapped by hybridisation to filters containing cosmid and P1 genomic libraries. The H2A.2 gene and the H2A.1-H2B.1 gene pair mapped between the ade6 and rik1 genes on chromosome III. The three H4-H3 gene pairs were mapped to three different regions by a H4.1 probe. Southern anal ysis of clones from each region revealed the positions of the three H4 -H3 gene pairs. H4.1-H3.1 was localised to chromosome I between the me i2 and rad1 genes; H4.2-H3.2 mapped between rad3 and cdc2 on chromosom e II; H4.3-H3.3 was localised to a region between the nuc1 and puc1 ge nes on chromosome II.