RAPID ISOLATION OF HIGHLY PRODUCTIVE RECOMBINANT CHINESE-HAMSTER OVARY CELL-LINES

Authors
MILOUX B LUPKER JH
Citation
B. Miloux et Jh. Lupker, RAPID ISOLATION OF HIGHLY PRODUCTIVE RECOMBINANT CHINESE-HAMSTER OVARY CELL-LINES, Gene, 149(2), 1994, pp. 341-344
Citations number
20
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
149
Issue
2
Year of publication
1994
Pages
341 - 344
Database
ISI
SICI code
0378-1119(1994)149:2<341:RIOHPR>2.0.ZU;2-S
Abstract
An expression system combining a unit for the expression of the gene o f interest reinforced by the hepatitis B virus X transactivator and a selectable gene weakened by the insertion of an A+T-rich sequence deri ved from the 3'-untranslated region of the granulocyte-macrophage colo ny-stimulating factor mRNA is described. This Vector allows rapid one- step isolation of highly productive Chinese hamster ovary clones.