Wc. Fick et Gj. Viljoen, EARLY AND LATE TRANSCRIPTIONAL PHASES IN THE REPLICATION OF LUMPY-SKIN-DISEASE VIRUS, Onderstepoort journal of veterinary research, 61(3), 1994, pp. 255-261
Approximately 71 of the estimated 145 kilobase pairs of the genome of
the South African isolate of lumpy-skin-disease virus, Neethling strai
n, was cloned into the plasmid vector pBluescribe. Selected clones wer
e used in Northern blot analysis to investigate the replication cycle
of the virus. The synthesis of early mRNA was initiated immediately af
ter infection, and continued for approximately 9 h. The transition to
late-gene transcription occurred approximately 10 h post-infection and
required DNA replication. Transcription of endogenous late genes cann
ot occur when viral DNA replication is prevented, while it is activate
d after DNA replication has occurred. The cloned fragments of the geno
me are expected to help in the identification of important promoter el
ements.