PH-INDEPENDENT INHIBITION OF RESTRICTION-ENDONUCLEASE CLEAVAGE VIA TRIPLE-HELIX FORMATION BY OLIGONUCLEOTIDES CONTAINING 8-OXO-2'-DEOXYADENOSINE

Authors
WANG Q TSUKAHARA S YAMAKAWA H TAKAI K TAKAKU H
Citation
Q. Wang et al., PH-INDEPENDENT INHIBITION OF RESTRICTION-ENDONUCLEASE CLEAVAGE VIA TRIPLE-HELIX FORMATION BY OLIGONUCLEOTIDES CONTAINING 8-OXO-2'-DEOXYADENOSINE, FEBS letters, 355(1), 1994, pp. 11-14
Citations number
23
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
355
Issue
1
Year of publication
1994
Pages
11 - 14
Database
ISI
SICI code
0014-5793(1994)355:1<11:PIORCV>2.0.ZU;2-T
Abstract
The ability of homopyrimidine oligonucleotides containing 8-oxo-2'-deo xyadenosine to form stable, triple helical structures with the sequenc e containing the recognition site for the class II-S restriction enzym e, Ksp632-I, was studied as a function of pH. The 8-oxo-2'-deoxyadenos ine-substituted oligomers were shown to inhibit enzymatic cleavage and to bind within the physiological pH range in a pH-independent fashion without compromising specificity.