DETERMINATION OF ALFUZOSIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN-SWITCHING

Authors
CARLUCCI G DIGIUSEPPE E MAZZEO P
Citation
G. Carlucci et al., DETERMINATION OF ALFUZOSIN IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH COLUMN-SWITCHING, Journal of liquid chromatography, 17(18), 1994, pp. 3989-3997
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
17
Issue
18
Year of publication
1994
Pages
3989 - 3997
Database
ISI
SICI code
0148-3919(1994)17:18<3989:DOAIHP>2.0.ZU;2-X
Abstract
A high-performance liquid chromatographic method for the determination of alfuzosin in human plasma has been developed and validated. A colu mn-switching procedure without extraction was used to isolate the drug from biological matrix prior to the quantitative analysis. The lower limit of detection for the analyte was 1 ng/ml. The method was linear from 2 to 150 ng/ml for human plasma. Within- and between-assay precis ion and accuracy were all found to be <5.2% at the eight concentration s evaluated. This procedure, simple and rapid, is suitable for pharmac ology studies on alfuzosin.