R. Kondo et al., BLEACHING OF HARDWOOD KRAFT PULP WITH MANGANESE PEROXIDASE SECRETED FROM PHANEROCHAETE SORDIDA YK-624, Applied and environmental microbiology, 60(12), 1994, pp. 4359-4363
In vitro bleaching of an unbleached hardwood kraft pulp was performed
with manganese peroxidase (MnP) from the fungus Phanerochaete sordida
YK-624. When the kraft pulp was treated with partially purified MnP in
the presence of MnSO4, Tween 80, and sodium malonate with continuous
addition of H2O2 at 37 degrees C for 24 h, the pulp brightness increas
ed by about 10 points and the kappa number decreased by about 6 points
compared with untreated pulp. The pulp brightness was also increased
by 43 points to 75.5% by multiple (six) treatments with MnP combined w
ith alkaline extraction. Our results indicate that in vitro degradatio
n of residual lignin in hardwood kraft pulp with MnP is possible.