POLYMERASE CHAIN-REACTION TYPING OF NONVIABLE MYCOBACTERIUM-TUBERCULOSIS ISOLATES

Authors
LOEFFELHOLZ MJ THOMPSON CJ GAUNT DD KOONTZ FP GILCHRIST MJR
Citation
Mj. Loeffelholz et al., POLYMERASE CHAIN-REACTION TYPING OF NONVIABLE MYCOBACTERIUM-TUBERCULOSIS ISOLATES, Diagnostic microbiology and infectious disease, 26(3-4), 1996, pp. 149-151
Citations number
9
Categorie Soggetti
Microbiology,"Infectious Diseases
Journal title
Diagnostic microbiology and infectious disease → ACNP
ISSN journal
07328893
Volume
26
Issue
3-4
Year of publication
1996
Pages
149 - 151
Database
ISI
SICI code
0732-8893(1996)26:3-4<149:PCTONM>2.0.ZU;2-#
Abstract
Repetitive element polymerase chain reaction (PCR) typing was applied to two Mycobacterium tuberculosis isolates for which both viable and n onviable cultures were available. DNA extracted from the nonviable cul tures and from fresh subcultures of the viable cultures was amplified with primers directed against the insertion sequence IS6110 and the po lymorphic GC-rich repetitive sequence. For both isolates, the nonviabl e cultures generated PCR-banding patterns identical to those generated by the fresh subcultures. (C) 1997 Elsevier Science Inc.