ECHINOCOCCUS-GRANULOSUS - THE MECHANISM OF ONCOSPHERE LYSIS BY SHEEP COMPLEMENT AND ANTIBODY

A heat-labile component of normal sheep serum (56 degrees C for 30 min but not 50 degrees C for 30 min) was able to lyse oncospheres in vitr o. The degree of effect, and the proportion of oncospheres lysed, was related to the concentration of normal unheated sheep serum complement , or other sources of complement (rabbit, mouse) in the culture. Lower concentrations were required for lysis if the culture serum was obtai ned from sheep immune to E. granulosus infection. Heat inactivation of normal or immune sheep serum removed any lytic ability. No lysis occu rred in any concentration of unheated foetal lamb serum. However, unhe ated foetal lamb serum was able to restore the lyric effect to heated normal or immune serum. This suggests that lysis in both immune and no rmal serum is antibody-dependent and complement-mediated. The lysis in normal serum would appear to be due to natural cross-reacting antibod ies that can fix complement at the oncosphere surface. The complement lesion resulted in damage to the plasma membrane. This then peeled bac k, predisposing the oncosphere to osmotic destruction. The use of blea ch to dissolve the embryophore caused damage to the plasma membrane si milar to that caused by complement. Developing metacestodes at 3 days of age in vitro in immune serum were susceptible to the addition of co mplement at that time.