A PCR STRATEGY FOR THE ISOLATION OF GLUTATHIONE S-TRANSFERASES (GSTS)FROM NEMATODES

Anchor based PCR technology has been used to isolate a GST sequence fr om the gastro-intestinal nematode Heligmosomoides polygyrus. A 800 bas e pair product was amplified from first-strand cDNA using primers base d on the N-terminal sequence of purified H. polygyrus GST (upstream pr imer) and a non-specific polyadenylate tail with an anchor sequence (d ownstream primer). The product was cloned into pUC18 and sequenced. A reading frame of 648 bases in the sequence encoded a protein which has 30% homology with the a family of mammalian glutathione S-transferase s.