EVALUATION OF NESTED POLYMERASE CHAIN METHODS FOR THE DETECTION OF HUMAN CORONAVIRUSES 229E AND OC43

Authors
MYINT S JOHNSTON S SANDERSON G SIMPSON H
Citation
S. Myint et al., EVALUATION OF NESTED POLYMERASE CHAIN METHODS FOR THE DETECTION OF HUMAN CORONAVIRUSES 229E AND OC43, Molecular and cellular probes, 8(5), 1994, pp. 357-364
Citations number
10
Categorie Soggetti
Cytology & Histology",Biology
Journal title
Molecular and cellular probes → ACNP
ISSN journal
08908508
Volume
8
Issue
5
Year of publication
1994
Pages
357 - 364
Database
ISI
SICI code
0890-8508(1994)8:5<357:EONPCM>2.0.ZU;2-C
Abstract
Currently, the diagnosis of human respiratory coronavirus infection is either stow or insensitive. This paper describes nested polymerase ch ain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determin ed and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell cultur e and may replace the latter as the diagnostic method of choice.