CHEMILUMINESCENT AND COLORIMETRIC DETECTION OF A FLUORESCEIN-LABELED PROBE AND A DIGOXIGENIN-LABELED PROBE AFTER A SINGLE HYBRIDIZATION STEP

The objective of the simple and fast method we describe is the simulta neous hybridization of two non-radioactive probes and their detection from the same blot, using two different systems. These two probes are synthesized by PCR: one is label led with fluorescein and the other wi th digoxigenin. The former is detected by chemiluminescence and the la tter by colorimetry. We applied this rapid and simple method to the sp ecific detection of multiplex polymerase chain reaction products. We u sed the human herpes simplex virus HSV1 and HSV2 PCR models studied in our laboratory.