POLY(ADP-RIBOSE) POLYMERASE GENE ON CHROMOSOME 1Q - EARLY ROLE IN DIFFERENTIATION LINKED REPLICATION - GENE ON HUMAN-CHROMOSOME 13Q - MARKER OF CARCINOGENESIS

We have focused in part on utilizing molecular aspects of the PADPRP g ene sequences located on chromosome Iq as well as on 13 to help clarif y the biological role of these genes. With respect to chromosome lq, s tably transfected cell lines have been established in-both HeLa as wel l as 3T3-L1 preadipocyte cells, which express a reverse complementary transcription product in vivo to the exons coded for on the chromosome 1 locus. Also, in the case of the PADPRP-like gene on chromosome 13q, we have fine mapped this sequence and have observed in a linkage of d eletion of this region with predisposition to various forms of cancer, especially in the African-American population.