IDENTIFICATION OF FUNCTIONAL PROMOTER ELEMENTS IN THE RABBIT SMOOTH-MUSCLE MYOSIN HEAVY-CHAIN GENE

Despite the importance of smooth muscle cell proliferation in vascular pathophysiological states, the mechanisms regulating smooth muscle ce ll growth and differentiation are poorly understood. Previous studies have shown that adult rabbit smooth muscles express two types of myosi n heavy chain (MHC) isoforms, SM1 and SM2, which are generated through alternative RNA splicing from a single smooth muscle MHC (SMHC) gene. In the present study, we isolated and characterized the rabbit SMHC g ene promoter. DNA sequence analysis of the upstream region of the SMHC gene revealed several putative cis-DNA regulatory elements proximal t o the transcription start site. Most notably, cis-acting regulatory el ements that closely resemble CC(A/T)(6)GG (CArG box) and myocyte enhan cer binding factor 2 (MEF-2)-type sequence motifs were found in the SM HC 5'-flanking region. In addition, six E-box motifs were found in the 5'-flanking region of the SMHC gene between -374 and -2109 base pairs from the transcription start site. A series of transient transfection assays using SMHC promoter deletion constructs indicated that a promo ter fragment extending to 2266 base pairs upstream of the transcriptio n start site has the highest reporter activity in cultured rat aortic smooth muscle cells. Gel mobility shift analyses using the MEF-2-like sequence located at -1540 revealed a specific DNA protein complex, whe reas the CArG-like element located at -1275 did not show protein bindi ng. The SMHC promoter construct, p509-CAT, which included neither the CArG- nor MEF-2-type motifs, conferred 32% of chloramphenicol acetyltr ansferase activity in the same cells, whereas the construct p188-CAT, which contained the minimal promoter elements (TATA box), was signific antly less active (7%; 2.0-fold over background). This is the first re port describing the promoter elements of a gene whose expression is re stricted to smooth muscle cells.