INDUCTION OF SEVERAL ANTIOXIDANT ENZYMES IN THE SELECTION OF A SALT-TOLERANT CELL-LINE OF PISUM-SATIVUM

Using the in vitro culture technique, we selected a cell line of Pisum sativum cv. Challis adapted to 85.5 mM NaCl. The possible relationshi ps between the activity of enzymes related to oxygen metabolism and th e salt adaptation of pea calli were analysed. The induction of two new Cu,Zn-SOD isozymes in salt-resistant calli was observed. Peroxidase a ctivity was greatly increased in selected calli and catalase did not s how a significant variation. The activity changes observed are discuss ed in terms of their possible relevance to pea calli adaptation to sal t-stress.