FRACTIONATION OF WHEAT, BARLEY, AND RYE PROLAMINS BY CATION-EXCHANGE FPLC

Wheat, rye, and barley prolamins were separated using optimized elutio n conditions by cation exchange FPLC on an analytical Mono-S column. W heat gliadins were resolved into a larger number of peaks than has bee n obtained hitherto. Most fractions contained a mixture of components as determined by SDS-PAGE and A-PAGE. At least one fraction was obtain ed for each group of gliadins that contains only one major component w ith minimal contamination by other components. Only omega-gliadins occ urred in five of the fractions. A preparative S-Sepharose Fast Flow co lumn, run under similar chromatographic conditions, was used to isolat e omega-gliadins. This procedure could be used as the first step for t he purification of individual gliadin components. Chromatography on th e Mono-S column was highly reproducible and showed promise as a means of differentiating wheat cultivars. Furthermore, FPLC was applied to f ractionate prolamins from barley and rye.