PROTOCOL FOR FAB MS MS CHARACTERIZATION OF TERPENE DISACCHARIDES OF WINE/

Application of fast atom bombardment tandem mass spectrometry (FAB MS/ MS) in positive and negative ion modes to mixtures of glycosides obtai ned from Riesling wine after DCCC, HPLC, and flash chromatography prov ided the molecular mass of the glycosides, the sequence of sugar attac hment, and information about the aglycons. Initial FAB MS/MS assignmen ts were supported by H-1 NMR data on isolated and derivatized glycosid es which allowed the characterization of E)-2,6-dimethyl-1-O-[apiofura nosyl-(1-->6)-beta-D- glucopyranosyl]-octa-2,6-ol and E)-2,6-dimethyl- 1-O-[apiofuranosyl-(1-->6)-beta-D- glucopyranosyl]-octa-2,6-dien-8-ol and the partial characterization of l-1-->6)-beta-D-glucopyranosyl]-p- menth-1-en-8-ol, and vomifoliol 9-O- arabinofuranosyl-D-glucopyranosid e.