ANEUPLOIDY IN ATYPICAL FIBROXANTHOMA - DNA CONTENT QUANTIFICATION OF 10 CASES BY IMAGE-ANALYSIS

It has recently been reported that atypical fibroxanthoma (AFX) is a p redominantly diploid lesion in contrast to malignant fibrous hystiocyt oma (MFH) which is usually aneuploid. To test this hypothesis, DNA con tent quantification was undertaken on Feulgen-stained cytology and tis sue section preparations from 10 cases of AFX by image analysis. The l arge atypical cells which characterize AFX were aneuploid in each case . Smaller spindle-shaped cells found in this lesion were diploid. The results suggest that AFX is indistinguishable from MFH by DNA content estimation and highlight an advantage of image analysis over flow cyto metry.