PRESENCE OF ANTITUMOR ACTIVITIES IN THE MILK COLLECTED FROM GOSSYPOL-TREATED DAIRY-COWS

Two human breast carcinoma cell lines (MCF-7, MCF-7 Adr) and a rat eso phageal cancer cell line (RE-B2T) were used to evaluate the antiprolif erative potential of gossypol (GP)-containing milk (GP-Milk), which wa s collected from Brown Swiss dairy cows treated daily with federally a llowable 450 ppm of GP for 6 days. Treatment of the cultured cancer ce lls with GP-Milk for 24 h significantly inhibited the rates of H-3-thy midine incorporation during the ensuing 3-h period in all three tumori genic cell lines. The inhibitory effects of GP-Milk occurred in a dose -dependent manner in all cases, but the calculated ED(50) varied with cell lines. ED(50) for GP-Milk was estimated at 10% for wild-type MCF- 7 human breast cancer cells, 15% for multidrug-resistant MCF-7 Adr hum an breast cancer cells and 50% for RE-B2T rat esophageal carcinoma cel ls. The potential of GP-Milk as a dietary supplement for the preventio n and/or treatment of human breast cancer is discussed in this paper.