ALKALINE-PHOSPHATASE AND PEPTIDASE ACTIVITIES IN CACO-2 CELLS - DIFFERENTIAL RESPONSE TO TRIIODOTHYRONINE

Authors
Citation
C. Jumarie et C. Malo, ALKALINE-PHOSPHATASE AND PEPTIDASE ACTIVITIES IN CACO-2 CELLS - DIFFERENTIAL RESPONSE TO TRIIODOTHYRONINE, In vitro cellular & developmental biology. Animal, 30A(11), 1994, pp. 753-760
Citations number
43
Categorie Soggetti
Developmental Biology","Cytology & Histology
ISSN journal
10712690
Volume
30A
Issue
11
Year of publication
1994
Pages
753 - 760
Database
ISI
SICI code
1071-2690(1994)30A:11<753:AAPAIC>2.0.ZU;2-6
Abstract
Caco-2 cell human colon adenocarcinoma cell line was used to study the hormonal regulation of small intestinal epithelial cell differentiati on. We had previously shown that insulin-transferrin-selenium and trii odothyronine (5 X 10(-8) M)-supplemented medium can best replace serum after 2 days of culture for both the maintenance and differentiation of Caco-2 cells. The present study demonstrates that precoating petri dishes with complete serum allows the growth and differentiation of Ca co-2 cells seeded directly in serum-free medium. On the other hand, pr ecoating with dialyzed serum inhibits alkaline phosphatase and dipepti dyl-dipeptidase IV activities by more than 50%. The results obtained w ith complete serum-precoatad culture plates indicate that there is no synergy between insulin and triiodothyronine because cells maintained in transferrin-selenium and triiodothyronine-supplemented medium, with or without insulin, express comparable enzyme activities. Moreover, l arge increases in alkaline phosphatase and dipeptidyl-dipeptidase IV a ctivities were observed when triiodothyronine was added to the culture medium by the time confluency was reached. In contrast, gamma-glutamy ltransferase was lowered to a greater extent when triiodothyronine was present from the beginning of culture. These findings show that triio dothyronine preferentially stimulates alkaline phosphatase and dipepti dyl-dipeptidase IV activities during the differentiation period wherea s it selectively inhibits gamma-glutamyltransferase during the prolife ration phase. Triiodothyronine acts in a dose-dependent manner.