J. Messiaen et P. Vancutsem, MEMBRANE, CYTOSOLIC, AND NUCLEAR RESPONSES INDUCED BY OLIGOGALACTURONIDES IN SUSPENSION-CULTURED CARROT CELLS, Journal of trace and microprobe techniques, 12(4), 1994, pp. 231-238
Oligogalacturonides were applied at a concentration of 0.2 mg m(-1) to
Daucus carota cells and protoplasts. Fractions containing oligomers o
f degree of polymerisation greater than or equal to 9 in the presence
of 150 mM NaCl and 0.5 mM CaCl2 (''high calcium'' conditions) triggere
d a cytosolic calcium mobilisation, membrane depolarisation and cytoso
lic acidification. These membrane and cytosolic events coincided with
the accumulation of transcripts encoding phenylalanine ammonialyase an
d catalytic activation of the enzyme. The effect of signal transductio
n modulators were investigated on oligogalacturonide-induced phenylala
nine ammonia-lyase enzyme and gene activation in suspension-cultured c
arrot cells.