STUDYING PLANT-CELL WALL BY IONIC MICROSCOPY - MAIN PROBLEMS ENCOUNTERED

Authors
JAUNEAU A RIPOLL C THELLIER M
Citation
A. Jauneau et al., STUDYING PLANT-CELL WALL BY IONIC MICROSCOPY - MAIN PROBLEMS ENCOUNTERED, Journal of trace and microprobe techniques, 12(4), 1994, pp. 295-303
Citations number
19
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of trace and microprobe techniques → ACNP
ISSN journal
07334680
Volume
12
Issue
4
Year of publication
1994
Pages
295 - 303
Database
ISI
SICI code
0733-4680(1994)12:4<295:SPWBIM>2.0.ZU;2-G
Abstract
The main processes involved in ionic microscopy are the production of the beam of primary ions, and the sputtering and ionization of the sam ple material. Possible difficulties or risks of artefacts are pointed out. The most difficult part of the method to image mobile substances such as metal cations in plant cell walls, is the preparation of the p lant samples. The performances of conventional tissue fixation, of pre cipitation by pyroantimonate and of cryomethods are compared with one another.