C. Levallois et al., NEUROPROTECTIVE EFFECT OF N-METHYL-D-ASPARTATE NONCOMPETITIVE ANTAGONISTS (ARYLCYCLOHEXYLAMINE DERIVATIVES) ON HUMAN CULTURED SPINAL-CORD CELLS, International journal of developmental neuroscience, 12(6), 1994, pp. 547-555
The non-competitive N-methyl-D-aspartate antagonist cis-Pip/Me 1-[1-(2
-thienyl)-2-methyl-cyclohexyl] piperidine (GK11) either in racemic(+/-
) or (-) enantiomeric form has been investigated on survival of human
fetal spinal cord cells in culture. The treated cultured cells were pr
ocessed for immunocytochemical detection of GABA at different time int
ervals ranging from 3 to 27 weeks. The number of GABA-stained cells wa
s found to be greater in the treated cultures than in the control ones
. The qualitative and quantitative morphological features of both cont
rol and treated cells were analysed by computer-assisted methods which
allowed us to individualize three different populations: (1) all cont
rol and treated small neuritic field neurons, (2) young control and ol
d treated large neuritic field neurons and (3) old control neurons wit
h large neuritic fields.