IMMOBILIZATION OF HUMAN THROMBOMODULIN ON BIOMATERIALS - EVALUATION OF THE ACTIVITY OF IMMOBILIZED HUMAN THROMBOMODULIN

Thrombomodulin (TM) is a newly described endothelial cell associated p rotein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant. In this stu dy, the immobilization of hTM was investigated in detail using surface modified polymers. As the basis of immobilization, poly(acrylic acid) (PAAc) surface-grafted poly(ethylene) (PAAc-g-PE) film was used with the expectation of increasing the immobilization amount of hTM. The ef fect of the immobilization reaction on the hTM activities, and the com parison of the activities of the immobilized hTM with the free hTM, we re studied.