NOVEL METHODS FOR THE PREPARATION OF ANTIBODY MICROPROBES

Antibody microprobes art devices which have been used to determine the release sites of several neuropeptides. The production of microprobes using the previous methodology is a time-consuming procedure requirin g a high level of skill. The aim of the study was to investigate alter native methods for the production of microprobes which would simplify this procedure. Specific antibodies to thyrotrophin-releasing hormone (TRH) were bound to the outside of glass and tungsten wire microprobes using (a) an aminosilane coating, (b) a polycarbonate plastic coating and (c) an epoxylite resin-activated charcoal coating. Microprobes we re assessed in vitro and in vivo for their ability to measure the neur opeptide TRH. All methods considerably reduced the time required to ma nufacture microprobes (more than 5-fold) and the aminosilane and epoxy lite resin/charcoal-coated probes were suitable for use in vivo. The p referred binding coat was epoxylite/charcoal which has now been used t o determine sites of TRH release in rat brain.