A. Fukamizu et al., NEUROECTODERMAL TUMORS EXPRESSING C-MYC, L-MYC, AND N-MYC IN TRANSGENIC MICE THAT CARRY THE E1A E1B GENE OF HUMAN ADENOVIRUS-TYPE-12/, The Journal of biological chemistry, 269(49), 1994, pp. 31252-31258
Adenovirus early 1 (E1) region gene products, including E1A and E1B, a
re required for transformation of primary cultured rodent cells. In or
der to investigate in vivo action of the E1 region, we established a l
ine of transgenic mice carrying the oncogenic E1A and E1B genes of hum
an adenovirus type 12 under control of the human angiotensinogen promo
ter. Histopathological analyses indicated that transgenic mice heritab
ly develop neuroectodermal tumors arising from the pelvic region with
varying degrees of incidence. The transgene was expressed in the neuro
ectodermal tumors as well as in TNT-1 cells, a cell line established f
rom the tumors, where the human angiotensinogen promoter was constitut
ively active. The high level expression of c-, L-, and N-myc without g
ene amplification was notable in the original tumors and TNT-1 cells,
but not in another tissue examined. The co-expression of the three set
s of myc family genes in both the original tumors and the established
cell line provided the possibility that the target cells for transform
ation may belong to a specific cell type that expresses all these onco
genes during development.