RNA14 AND RNA15 PROTEINS AS COMPONENTS OF A YEAST PRE-MESSENGER-RNA 3'-END PROCESSING FACTOR

Citation
L. Minviellesebastia et al., RNA14 AND RNA15 PROTEINS AS COMPONENTS OF A YEAST PRE-MESSENGER-RNA 3'-END PROCESSING FACTOR, Science, 266(5191), 1994, pp. 1702-1705
Citations number
26
Categorie Soggetti
Multidisciplinary Sciences
Journal title
ISSN journal
00368075
Volume
266
Issue
5191
Year of publication
1994
Pages
1702 - 1705
Database
ISI
SICI code
0036-8075(1994)266:5191<1702:RARPAC>2.0.ZU;2-A
Abstract
Most eukaryotic pre-messenger RNAs are processed at their 3' ends by e ndonucleolytic cleavage and polyadenylation. In yeast, this processing requires polyadenylate [poly(A)] polymerase (PAP) and other proteins that have not yet been characterized. Here, mutations in the PAP1 gene were shown to be synergistically lethal with previously identified mu tations in the RNA14 and RNA15 genes, which suggests that their encode d proteins participate in 3'-end processing. Indeed, extracts from ma1 4 and ma15 mutants were shown to be deficient in both steps of process ing. Biochemical complementation experiments and reconstitution of bot h activities with partially purified cleavage factor I (CF I) validate d the genetic prediction.