The transformation of a mixture of water and wheat flour into a viscoe
lastic dough was studied by light microscopy, scanning and transmissio
n electron microscopy. In flour particles, gluten protein fills up the
space between the starch granules forming a three-dimensional network
. Dough formation is the result of the adhesion of the protein network
s of individual flour particles to one another and their extension dur
ing kneading. In the course of the kneading process protein films form
from the strands by bidirectional extension. The strands of the glute
n network consist of randomly arranged protein threads which are twist
ed to form loops and knots in the unextended state. Bidirectional exte
nsion of the gluten pulls apart these knots to platelet-like forms.