JASMONATE-INDUCED PROTEINS IN COTTON - IMMUNOLOGICAL RELATIONSHIP TO THE RESPECTIVE BARLEY PROTEINS AND HOMOLOGY OF TRANSCRIPTS TO LATE EMBRYOGENESIS ABUNDANT (LEA) MESSENGER-RNAS
S. Reinbothe et al., JASMONATE-INDUCED PROTEINS IN COTTON - IMMUNOLOGICAL RELATIONSHIP TO THE RESPECTIVE BARLEY PROTEINS AND HOMOLOGY OF TRANSCRIPTS TO LATE EMBRYOGENESIS ABUNDANT (LEA) MESSENGER-RNAS, Journal of plant growth regulation, 11(1), 1992, pp. 7-14
(-)-Jasmonic acid methyl ester (Ja-Me) causes a drastic alteration of
gene expression in excised cotton (Gossypium hirsutum L.) cotyledons.
After in vivo labeling with L-[S-35] methionine, soluble proteins of J
a-Me-treated cotyledonary segments were analyzed by two-dimensional po
lyacrylamide gel electrophoresis. Several different classes of polypep
tides were observed, corresponding to molecular sizes of Mr 67, 40, 35
, 30, 25, 23, 20, 18 kDa, which were absent in water-treated (control)
segments. Western-blot analysis with antibodies raised against 66, 37
, and 23 kDa jasmonate-induced proteins (JIPs) of barley (Herrmann et
al. 1989) revealed that only the 23 kDa JIPs of cotton are immunologic
ally related to those of barley. The Ja-Me-induced alteration in the p
rotein pattern correlated with the appearance of novel in vitro transl
atable mRNAs which accumulated transiently or steadily during the incu
bation period. Three of the Ja-Me-induced mRNAs hybridized with a synt
hetic oligonucleotide probe that was complementary to a highly conserv
ed sequence motif found in the cotton Lea (late embryogenesis abundant
) gD-7 gene (Baker et al. 1988) and in some other Lea genes (Dure et a
l. 1989). The level of the 2.17, 1.46, and 0.71 kb transcripts detecte
d at a melting temperature (Tm) -15-degrees-C criterion did not change
significantly in the water-control cotyledons, but they increased thr
ee- to fourfold in jasmonate-treated cotyledons.