ONCOGENE-DIRECTED MUTAGENESIS INVIVO - POLYALKYLATING DERIVATIVES OF SHORT SINGLE-STRANDED POLYNUCLEOTIDES COMPLEMENTARY TO THE E1-ADENOONCOGENE IN NORMALIZATION OF ADENOVIRUS-TRANSFORMED RODENT CELL-LINES

Polyadenylating derivatives of single-stranded polynucleotides with a length of 30-200 nucleotides, complementary to the long El oncogene se quences of simian adenovirus SA7, induce normalization of the phenotyp ic properties of the rodent cell lines SH2 and G11 transformed by aden ovirus SA7, in certain cases forming deletions in the ElA region of th e integrated proviral oncogene. The transformed cells are indifferent to reagents noncomplementary to the El region. Thus, polyalkylating de rivatives of single-stranded 30-200-mers are addressed mutagens, which in transformed rodent cells react highly specifically with integrated DNA sequences of the El oncogene complementary to them and implement oncogene-directed mutagenesis in vivo (according to the classification proposed earlier: B. Botstain and D. Shortle, Science, 229, 1193-1201 (1985); O. K. Shulyugin and I. Fodor, Biotekhnologiya, 3-8 (1990)]. T emporarily normalized cells, which revert to the original transformed phenotype during passage, are also formed in this case.