An immunohistochemical study of non-small cell lung carcinoma using PC
1O, a monoclonal antibody against PCNA, was performed on tissues routi
nely processed with formalin fixation and paraffin embedding. The PCNA
labelling index and mitotic index were determined from sections of th
ese tissues. Tumours showed a high mean PCNA labelling index of 53.3%.
The mean mitotic index was 10.3/1000 cells. Inter-examiner agreement
of mitotic counting was good. A linear correlation between the PCNA la
belling index and mitotic index was demonstrated (r = 0.71, P < 0.0000
1). It is concluded that immunohistochemical nuclear labelling with an
ti-PCNA on routinely processed tissue is a simple technique for the as
sessment of proliferation in non-small cell lung carcinoma.