RNASE-H ACTIVITY OF HIV REVERSE TRANSCRIPTASES IS CONFINED EXCLUSIVELY TO THE DIMERIC FORMS

Authors
RESTLE T MULLER B GOODY RS
Citation
T. Restle et al., RNASE-H ACTIVITY OF HIV REVERSE TRANSCRIPTASES IS CONFINED EXCLUSIVELY TO THE DIMERIC FORMS, FEBS letters, 300(1), 1992, pp. 97-100
Citations number
21
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
300
Issue
1
Year of publication
1992
Pages
97 - 100
Database
ISI
SICI code
0014-5793(1992)300:1<97:RAOHRT>2.0.ZU;2-V
Abstract
A method for the rapid preparation of a defined substrate to monitor R Nase H activity has been developed. Using this substrate, we have inve stigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse transcriptase (RT) in detail. As we report her e, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes