CONTROL OF COLUMN INFLUENCE ON THE WIDE-RANGE PH GRADIENT IN ION-EXCHANGE CHROMATOGRAPHY

Authors
SLAIS K FRIEDL Z
Citation
K. Slais et Z. Friedl, CONTROL OF COLUMN INFLUENCE ON THE WIDE-RANGE PH GRADIENT IN ION-EXCHANGE CHROMATOGRAPHY, Chromatographia, 33(5-6), 1992, pp. 231-236
Citations number
15
Journal title
Chromatographia → ACNP
ISSN journal
00095893
Volume
33
Issue
5-6
Year of publication
1992
Pages
231 - 236
Database
ISI
SICI code
0009-5893(1992)33:5-6<231:COCIOT>2.0.ZU;2-J
Abstract
A method for optimization of the mobile phase composition in ion excha nge chromatography with a wide range external pH gradient (approx. ove r 5 pH units) has been formulated. It is shown that programming of var ious wide range pH profiles which are not impaired by buffer interacti ons of the mobile phase with sorbent is possible. Utilization of the w ide range external pH gradient in cation-exchange chromatography of bo vine haemoglobin is also demonstrated.