Ia. Roewer et al., TRANSIENT INDUCTION OF TRYPTOPHAN DECARBOXYLASE (TDC) AND STRICTOSIDINE SYNTHASE (SS) GENES IN CELL-SUSPENSION CULTURES OF CATHARANTHUS-ROSEUS, Plant cell reports, 11(2), 1992, pp. 86-89
When cell suspension cultures of Catharanthus roseus are treated with
autoclaved elicitor from the fungus Pythium aphanidermatum, they respo
nd with the rapid transient induction of tryptophan decarboxylase (TDC
) and strictosidine synthase (SS) enzyme activities, followed by the a
ccumulation of indole alkaloids (Eilert et al., 1987). In this report,
we demonstrate that expression of TDC and SS enzyme activities is pre
ceeded by the transient appearance of mRNAs for both enzymes, suggesti
ng transcriptional control of these events. The strong transient accum
ulation of both TDC and SS enzyme transcripts observed in elicitor-tre
ated cell suspension cultures contrasts with the barely detectable lev
el of TDC transcripts and the undetectable level of SS transcripts obs
erved in developing seedlings.