THERMODYNAMICS OF ENZYMATIC PEPTIDE-SYNTHESIS IN BIPHASIC AQUEOUS-ORGANIC SYSTEMS - COUPLING OF THE CHEMICAL AND PHASE-EQUILIBRIA

Authors
IVANOV IP KASCHE V PETKOV DD
Citation
Ip. Ivanov et al., THERMODYNAMICS OF ENZYMATIC PEPTIDE-SYNTHESIS IN BIPHASIC AQUEOUS-ORGANIC SYSTEMS - COUPLING OF THE CHEMICAL AND PHASE-EQUILIBRIA, Biocatalysis and biotransformation, 14(3), 1997, pp. 181-194
Citations number
21
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biocatalysis and biotransformation → ACNP
ISSN journal
10242422
Volume
14
Issue
3
Year of publication
1997
Pages
181 - 194
Database
ISI
SICI code
1024-2422(1997)14:3<181:TOEPIB>2.0.ZU;2-U
Abstract
Chemical and phase equilibria in enzymic peptide synthesis in a biphas ic aqueous-organic system are coupled, the mass transfer providing the ''driving force'' for the synthetic reaction. The overall synthetic e quilibrium is characterized by an equilibrium constant, independent of pH, phase volume ratio and reactants partition coefficients. A linear free-energy relationship is observed for this equilibrium constant an d the extraction equilibrium constant.