DETERMINATION OF NICOTINE IN SERUM BY TLC-DENSITOMETRY

Authors
LIAKOPOULOUKYRIAKIDES M PLATIS F MOUTSOS NB KORTSARIS A
Citation
M. Liakopouloukyriakides et al., DETERMINATION OF NICOTINE IN SERUM BY TLC-DENSITOMETRY, Analytical letters, 25(3), 1992, pp. 485-496
Citations number
15
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
25
Issue
3
Year of publication
1992
Pages
485 - 496
Database
ISI
SICI code
0003-2719(1992)25:3<485:DONISB>2.0.ZU;2-E
Abstract
A TLC-Densitometric procedure for determination of nicotine in serum i s reported. Nicotine was extracted with ethylacetate from protein free serum almost quantitatively and evaporated to dryness with a stream o f nitrogen. The residue was oxidized to nicotinic acid with concentrat ed nitric acid, diluted with methanol to a standard volume and then de termined quantitatively by TLC-densitometer at lambda(max)262 nm.