CYTOCHEMICAL STUDIES ON SPERM SUCCINATE-DEHYDROGENASE AMONG CATTLE AND BUFFALOS

Authors
BENJAMIN BR MOHAN G VERMA RP
Citation
Br. Benjamin et al., CYTOCHEMICAL STUDIES ON SPERM SUCCINATE-DEHYDROGENASE AMONG CATTLE AND BUFFALOS, Indian Journal of Animal Sciences, 62(1), 1992, pp. 28-30
Citations number
NO
Journal title
Indian Journal of Animal Sciences → ACNP
ISSN journal
03678318
Volume
62
Issue
1
Year of publication
1992
Pages
28 - 30
Database
ISI
SICI code
0367-8318(1992)62:1<28:CSOSSA>2.0.ZU;2-V
Abstract
Cytochemical evaluation was conducted on sperm succinate dehydrogenase (SD) in crossbred, Holstein and Murrah buffalo. The enzymic action on disodium succinate hexahydrate (mol. wt 270) and disodium succinate ( mol. wt 162) were tried at 0.2M and 0.5M concentrations. The disodium succinate (anhydrous) yielded better results for the cytochemical appr aisal of SD. Also, the other sperm reductases confounding the assessme nt of SD in situ, were better blocked by desiccation (for nearly 10 mi n at room temperature) than the heat treatment (at 50-degrees-C for 30 min).