CHARACTERIZATION AND DETOXIFICATION OF AN EASILY PREPARED ACELLULAR PERTUSSIS-VACCINE - ANTIGENIC ROLE OF THE A-PROTOMER OF PERTUSSIS TOXIN

Authors
GARCIASAINZ JA ROMEROAVILA MT RUIZARRIAGA A RUIZPUENTE J AGUNDIS C ORTIZ V ISIBASI A
Citation
Ja. Garciasainz et al., CHARACTERIZATION AND DETOXIFICATION OF AN EASILY PREPARED ACELLULAR PERTUSSIS-VACCINE - ANTIGENIC ROLE OF THE A-PROTOMER OF PERTUSSIS TOXIN, Vaccine, 10(5), 1992, pp. 341-344
Citations number
19
Journal title
Vaccine → ACNP
ISSN journal
0264410X
Volume
10
Issue
5
Year of publication
1992
Pages
341 - 344
Database
ISI
SICI code
0264-410X(1992)10:5<341:CADOAE>2.0.ZU;2-8
Abstract
An acellular pertussis vaccine was prepared from pertussis vaccine con centrates by eliminating the cells by centrifugation and subsequent ac id precipitation. SDS-PAGE analysis indicated the presence of 17 clear ly defined bands including the subunits of pertussis toxin. The acellu lar preparation was detoxified with glutaraldehyde and lost its undesi rable effects (leukocytosis-promoting activity, histamine-sensitizing activity and decrease in weight gain) but retained a very good protect ive activity (PD50 almost-equal-to 2-mu-g protein/mouse). Sera from mi ce immunized with this preparation indicated an antibody response agai nst the A protomer of pertussis toxin.