BACILYSIN PRODUCTION BY BACILLUS-SUBTILIS - EFFECTS OF BACILYSIN, PH AND TEMPERATURE

The dipeptide antibiotic bacilysin, when added externally to the early exponential-phase cultures, markedly limited its own synthesis. It wa s shown in cell-free extracts that the feedback effect does not involv e the inhibition of bacilysin synthetase, the enzyme catalyzing bacily sin formation. We also studied pH and temperature dependence of bacily sin production. Production was highest at about pH 6.8 and at 25-degre es-C.