INTERACTIONS OF 3'-TERMINAL AND 5'-TERMINAL REGIONS OF PHYSALIS MOTTLE VIRUS GENOMIC RNA WITH ITS REPLICATION COMPLEX

Citation
V. Srividhya et Hs. Savithri, INTERACTIONS OF 3'-TERMINAL AND 5'-TERMINAL REGIONS OF PHYSALIS MOTTLE VIRUS GENOMIC RNA WITH ITS REPLICATION COMPLEX, Journal of Biosciences, 21(6), 1996, pp. 743-753
Citations number
25
Categorie Soggetti
Biology
Journal title
ISSN journal
02505991
Volume
21
Issue
6
Year of publication
1996
Pages
743 - 753
Database
ISI
SICI code
0250-5991(1996)21:6<743:IO3A5R>2.0.ZU;2-#
Abstract
Physalis mottle virus (PhMV) belongs to the tymogroup of positive-stra nd RNA viruses with a genome size of 6 kb. Crude membrane preparations from PhMV-infected Nicotiana glutinosa plants catalyzed the synthesis of PhMV genomic RNA from endogenously bound template. Addition of exo genous genomic RNA enhanced the synthesis which was specifically inhib ited by the addition of sense and antisense transcripts corresponding to 3' terminal 242 nucleotides as well as the 5' terminal 458 nucleoti des of PhMV genomic RNA while yeast tRNA or ribosomal RNA failed to in hibit the synthesis. This specific inhibition suggested that the 5' an d 3' non-coding regions of PhMV RNA might play an important role in vi ral replication.