Optimal environmental conditions were determined for stage II micropro
pagation of the sugarcane cultivar H62-4671. The optimum conditions ba
sed on the cost of production were found to be a temperature of 31-deg
rees-C, a medium sucrose content of 2%, 6 initial shoots per vessel, a
photosynthetic photon flux of 200-mu-mol/m2.s, and three medium chang
es over the 14 day growth period. However, the maximum plant growth wa
s with two initial shoots per vessel, a photosynthetic photon flux of
310-mu-mol/m2.s, and four medium changes. No significant increases in
growth due to ventilation, carbon dioxide enrichment, ethylene enrichm
ent, or dew point depression were found.