GENETIC-TRANSFORMATION OF ASPEN (POPULUS-TREMULAXPOPULUS-ALBA) BY AGROBACTERIUM-RHIZOGENES AND REGENERATION OF PLANTS TOLERANT TO HERBICIDE

Authors
DEVILLARD C
Citation
C. Devillard, GENETIC-TRANSFORMATION OF ASPEN (POPULUS-TREMULAXPOPULUS-ALBA) BY AGROBACTERIUM-RHIZOGENES AND REGENERATION OF PLANTS TOLERANT TO HERBICIDE, Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie, 314(6), 1992, pp. 291-298
Citations number
16
Journal title
Comptes rendus de l'Academie des sciences. Serie 3, Sciences de la vie → ACNP
ISSN journal
07644469
Volume
314
Issue
6
Year of publication
1992
Pages
291 - 298
Database
ISI
SICI code
0764-4469(1992)314:6<291:GOA(BA>2.0.ZU;2-P
Abstract
An oncogenic strain of Agrobacterium rhizogenes, containing the bar ge ne which confers resistance to the herbicide Basta, was used for trans formation of an aspen hybrid (Populus tremula x Populus alba). From 28 to 54% of inoculated stem sections formed roots after inoculation by the bacteria according to methods of inoculation. Plants were regenera ted from transformed roots. Successful genetic transformation was conf irmed by enzyme assays and Southern blot analysis. Transgenic plants t ransferred to soil and sprayed in the greenhouse showed tolerance towa rds Basta.