NUCLEOTIDE-SEQUENCES OF CDNA CLONES, PSV-1 AND PSV-2, HYBRIDIZING TO ANDROGEN-STIMULATED MESSENGER-RNAS IN RAT SEMINAL-VESICLES

In order to further characterize the cDNA clones, pSv-1 and pSv-2, whi ch had been newly isolated from a cDNA library of intact rat seminal v esicles as clones hybridizing to androgen-stimulated mRNAs of approxim ately 1,500 and 3,500 nucleotides in length, respectively, the whole n ucleotide sequences were determined. The pSv-1 and pSv-2 were 1135 and 1819 nucleotides in length, respectively, and seemed not to contain e ntire sequences corresponding to the mRNAs. When the 0.7 kb HindIII fr agments from pSv-1 and the 1 kb fragments from pSv-2 were used to prob e rat genomic DNA that had been digested with four restriction enzymes , the Southern blots suggested the existence of multiple genes related to pSv-1 and a single gene related to pSv-2. These results suggest th at pSv-1 and pSv-2 provide useful probes not only for further characte rization of products encoded by the mRNAs, but also for the study on t he physiological roles of androgen-dependent gene expression in rat se minal vesicles.